LYSOSOMAL ENZYMES IN AQUATIC SPECIES .I. DISTRIBUTION AND PARTICLE PROPERTIES OF MUSCLE LYSOSOMES OF GOLDFISH

被引：35

作者：

BIRD, JWC

BERG, T

MILANESI, A

STAUBER, WT

机构：

[1] Department of Physiology, Nelson Biological Laboratories, Rutgers University, New Brunswick

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY | 1969年 / 30卷 / 03期

关键词：

acid ribonuclease; aryl sulfatase; Carassius auratus; cathepsin-D; fish; goldfish; Lysosome; mitochondria; muscle; β-glucuronidase;

D O I：

10.1016/0010-406X(69)92015-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Procedures have been established for the quantitative assay of cathepsin D, acid ribonuclease, aryl sulfatase and β-glucuronidase in the white muscle of the goldfish. 2. 2. These acids hydrolases occur in partly latent and sedimentable form and are rendered soluble by treatments which liberate the enzymes from rat-liver lysosomes. 3. 3. Fractionation of total homogenates by differential centrifugation shows that the particulate enzymes have their greatest concentration in the light mitochondrial fraction. 4. 4. Fish muscle is shown to be quite favorable for the study of lysosome function in muscle tissue. © 1969.

引用

页码：457 / +

页数：1

共 36 条

[1] The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin [J].