RAPID DETECTION OF HEPATITIS-C VIRUS-RNA BY DIRECT CAPTURE FROM BLOOD

被引：16
作者：
VANDOORN, LJ
KLETER, B
VOERMANS, J
MAERTENS, G
BROUWER, H
HEIJTINK, R
QUINT, W
机构：
[1] ERASMUS UNIV ROTTERDAM,DEPT VIROL,3000 DR ROTTERDAM,NETHERLANDS
[2] INNOGENET NV,B-9052 GHENT,BELGIUM
[3] DIJKZIGT ACAD HOSP,DEPT INTERNAL MED 2,3015 GD ROTTERDAM,NETHERLANDS
来源：
JOURNAL OF MEDICAL VIROLOGY | 1994年 / 42卷 / 01期
关键词：
HCV-RNA; HCV DIAGNOSIS; PCR; MAGNETIC PARTICLES;
D O I：
10.1002/jmv.1890420105
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new diagnostic assay for hepatitis C virus RNA detection is described. HCV genomic RNA is captured onto streptavidin-coated magnetic beads by solution hybridization with biotinylated complementary oligonucleotides. The specificity of the capture assay is confirmed using different capture oligonucleotides as well as sera representing different types of HCV. Sensitivity was determined by testing serial dilutions of a HCV infected plasma. A panel of 50 sera was tested for anti-HCV by a Line Immunoassay and for HCV-RNA by both a conventional guanidinium extraction method and the new capture assay. The specificity of the capture assay was 95.8% and the sensitivity was 92.3% compared to the standard protocol. This method provides a rapid and simple alternative for HCV-RNA detection in blood samples.
引用
收藏
页码：22 / 28
页数：7
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