A CONTROL MODEL FOR TIBIAL CORTEX NEOVASCULARIZATION IN THE BONE CHAMBER

被引:44
作者
WINET, H
BAO, JY
MOFFAT, R
机构
[1] Department of Orthopedics, University of Southern California, Orthopedic Hospital, Los Angeles, California
关键词
D O I
10.1002/jbmr.5650050106
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Neovascularization across a gap defect in a rabbit tibial cortex was monitored using the optical bone chamber implant (BCI). Cortical bone growing by apposition as trabeculae was observed weekly as it penetrated a slit into a tissue space in vivo and in situ. Each rabbit was viewed weekly with an intravital microscope from 3 to 8 weeks postimplantation. The constant field of view was the slit‐gap tissue space, which was 100 μm thick and 2 mm in diameter. Vessels were imaged with epi‐illuminated fluorescence microscopy as they carried FITC‐dextran 70 that had been injected into an aural vein. Observations were videotaped and photographed. Videotape frames were analyzed with a digital image processing system to obtain measures of vessel length per unit volume (L/V) of fibroblastic granular tissue and trabeculae, caliber C, and flow velocity u, all as functions of time. Observations supported the conclusions that (1) neovascularization precedes neo‐osteogenesis, (2) major vessels tend to align with the tibial axis, (3) bone apposition‐generated destruction of fibrous granular tissue vessels stimulates fibrous granular tissue angiogenesis, which keeps its L/V constant, (4) L/V in trabeculae increases with time, and (5) blood supply (Q̄) and nutrient exchange in healing trabeculae are not positively correlated. Thus, O2 supply to the trabeculum cannot be predicted from Q̄ alone because the nutrient exchange area is not constant. It was noted that an increase in the potential nutrient exchange area occurred in both fibrous granular tissue and osseous vessels and the volume fraction of blood decreased in the fibrous granular tissue and remained constant in the trabeculae Copyright © 1990 ASBMR
引用
收藏
页码:19 / 30
页数:12
相关论文
共 28 条
[1]  
AKHNAZAROVA S, 1982, EXPT OPTIMIZATION CH, P56
[2]   MICROCIRCULATION IN GRAFTED BONE - CHAMBER TECHNIQUE FOR VITAL MICROSCOPY OF RABBIT BONE TRANSPLANTS [J].
ALBREKTSSON, T ;
ALBREKTSSON, B .
ACTA ORTHOPAEDICA SCANDINAVICA, 1978, 49 (01) :1-7
[3]  
ALBREKTSSON T, 1987, CRIT REV BIOCOMPAT, V3, P25
[4]  
ALBREKTSSON T, 1979, THESIS U GOTEBORG SW
[5]  
BAGGE U, 1984, COMMUNICATION
[6]  
BRANEMAR.PI, 1964, ACTA ANAT, V59, P1
[7]  
BRANEMARK PI, 1958, LUNDS U ARSSKR, V24, P1
[8]  
CHARM SE, 1974, BLOOD FLOW MICROCIRC, P18
[9]   Microscopic observations on new formation of cartilage and bone in the living mammal [J].
Clark, ER ;
Clark, EL .
AMERICAN JOURNAL OF ANATOMY, 1942, 70 (02) :167-200
[10]   MICRO-CIRCULATORY FLOW CHANGES DURING TISSUE-GROWTH [J].
DUDAR, TE ;
JAIN, RK .
MICROVASCULAR RESEARCH, 1983, 25 (01) :1-21