DETECTION OF MULTIPLE VIRULENCE-ASSOCIATED GENES OF LISTERIA-MONOCYTOGENES BY PCR IN ARTIFICIALLY CONTAMINATED MILK SAMPLES

被引：60

作者：

COORAY, KJ

NISHIBORI, T

XIONG, HB

MATSUYAMA, T

FUJITA, M

MITSUYAMA, M

机构：

[1] NIIGATA UNIV, SCH MED, DEPT BACTERIOL, NIIGATA 951, JAPAN

[2] INST MED RES, DEPT FOOD & WATER BACTERIOL, COLOMBO, SRI LANKA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 08期

关键词：

D O I：

10.1128/AEM.60.8.3023-3026.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.

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页码：3023 / 3026

页数：4

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