INTERACTION OF PLASMA GELSOLIN WITH TROPOMYOSIN

被引：23

作者：

KOEPF, EK ^{[1
]}

BURTNICK, LD ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT CHEM,VANCOUVER V6T 1Z1,BC,CANADA

来源：

FEBS LETTERS | 1992年 / 309卷 / 01期

关键词：

GELSOLIN; TROPOMYOSIN;

D O I：

10.1016/0014-5793(92)80738-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin-agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein-5-isothiocyanate. Both of these effects require the presence of calcium ions.

引用

页码：56 / 58

页数：3

共 17 条

[1]

Bagshaw C. R., 1987, SPECTROPHOTOMETRY SP, P91

[2] MODIFICATION OF ACTIN WITH FLUORESCEIN ISOTHIOCYANATE [J].

BURTNICK, LD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 791 (01) :57-62

[3] INTERACTION OF MUSCLE AND NON-MUSCLE TROPOMYOSINS WITH DEOXYRIBONUCLEASE-I [J].

CLARK, ID ;

BURTNICK, LD .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 185 (01) :105-109

[4] FLUORESCENCE OF EQUINE PLATELET TROPOMYOSIN LABELED WITH ACRYLODAN [J].

CLARK, ID ;

BURTNICK, LD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 260 (02) :595-600

[5]

COTE GP, 1983, MOL CELL BIOCHEM, V57, P126

[6]

ISHIKAWA R, 1989, J BIOL CHEM, V264, P7490

[7]

JANMEY PA, 1987, BLOOD, V70, P524

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

LEE WM, 1992, NEW ENGL J MED, V326, P1335

[10] THE THIN-FILAMENTS OF SMOOTH MUSCLES [J].

MARSTON, SB ;

SMITH, CWJ .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1985, 6 (06) :669-708

← 1 2 →