ENHANCED INSULIN-LIKE GROWTH FACTOR-I GENE-EXPRESSION IN REGENERATING RAT PANCREAS

被引：118

作者：

SMITH, FE ^{[1
]}

ROSEN, KM ^{[1
]}

VILLAKOMAROFF, L ^{[1
]}

WEIR, GC ^{[1
]}

BONNERWEIR, S ^{[1
]}

机构：

[1] HARVARD UNIV, CHILDRENS HOSP, SCH MED, DEPT NEUROL, BOSTON, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 14期

关键词：

SOMATOMEDIN-C; PANCREATIC BETA CELL; PANCREATIC DUCTULES; INSITU HYBRIDIZATION;

D O I：

10.1073/pnas.88.14.6152

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Insulin-like growth factor I (IGF-I) mRNA expression was studied after 90% partial pancreatectomy in the rat to determine whether IGF-I was associated with pancreatic regeneration. The level of IGF-I mRNA was maximally increased (4-fold above control value) 3 days after pancreatectomy, but thereafter gradually decreased, returning to control levels by 14 days after surgery. By in situ hybridization, IGF-I mRNA in both pancreatectomized and sham-operated rats was localized to capillary endothelial cells, indicating that this is the site of IGF-I expression in the normal rat pancreas. However, enhanced IGF-I mRNA expression was localized to focal areas of regeneration unique to pancreatectomized rats. In these areas, epithelial cells of proliferating ductules and individual connective tissue cells expressed IGF-I, suggesting that IGF-I may play an important role in the growth or differentiation of pancreatic tissue.

引用

页码：6152 / 6156

页数：5

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