METABOLISM OF BENZO[A]PYRENE AND 7,12-DIMETHYLBENZ[A]ANTHRACENE IN CULTURED HUMAN-FETAL AORTIC SMOOTH-MUSCLE CELLS

被引：46

作者：

BOND, JA ^{[1
]}

KOCAN, RM ^{[1
]}

BENDITT, EP ^{[1
]}

JUCHAU, MR ^{[1
]}

机构：

[1] UNIV WASHINGTON, SCH MED, DEPT PATHOL, SEATTLE, WA 98195 USA

来源：

LIFE SCIENCES | 1979年 / 25卷 / 05期

关键词：

D O I：

10.1016/0024-3205(79)90574-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Cultured human fetal aortic smooth muscle cells derived from the abdominal aorta converted benzo[a]pyrene (BaP) and 7,12-dimethylbenz[a]anthracene (DMBA) via cytochrome P-450-dependent monooxygenation to metabolites detectable by both a highly sensitive radiometric assay and high pressure liquid chromatography (HPLC). Cells incubated with 3H-BaP transformed this substrate primarily to phenols. 14C-DMBA was converted to metabolites that cochromatographed with 12-hydroxymethyl-7-methylbenz[a]anthracene, 7-hydroxymethyl-12-methylbenz-[a]anthracene, 7,12-dihydroxymethylbenz[a]anthracene, and trans-8,9-dihydrodiol-7,12-DMBA. Exposure of cells in culture to 13 μM 1,2-benz[a]anthracene resulted in increased oxidative metabolism of both BaP and DMBA. In the case of BaP, total phenol formation was increased, while with DMBA all metabilities detected by HPLC were increased. Support for the potential role of metabolism of polycyclic aromatic hydrocarbons by aortic smooth muscle cells in the etiology of atherosclerosis was obtained. © 1979.

引用

页码：425 / 430

页数：6

共 13 条

[1]

ALBERT RE, 1977, CANCER RES, V37, P2232

[2] EVIDENCE FOR A MONOCLONAL ORIGIN OF HUMAN ATHEROSCLEROTIC PLAQUES [J].

BENDITT, EP ;

BENDITT, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (06) :1753-1756

[3] ORIGIN OF ATHEROSCLEROSIS [J].

BENDITT, EP .

SCIENTIFIC AMERICAN, 1977, 236 (02) :74-&

[4] EVIDENCE FOR A MONOCLONAL ORIGIN OF HUMAN ATHEROSCLEROTIC PLAQUES AND SOME IMPLICATIONS [J].

BENDITT, EP .

CIRCULATION, 1974, 50 (04) :650-652

[5]

BENDITT EP, INT REV EXP PATH

[6] KINETICS, ACTIVATION, AND INDUCTION OF AORTIC MONO-OXYGENASES - BIOTRANSFORMATION OF BENZO[A]PYRENE [J].

BOND, JA ;

OMIECINSKI, CJ ;

JUCHAU, MR .

BIOCHEMICAL PHARMACOLOGY, 1979, 28 (02) :305-311

[7]

DIGIOVANNI J, 1977, DRUG METAB DISPOS, V5, P295

[8] ULTRASTRUCTURAL AND IMMUNOHISTOCHEMICAL STUDIES OF PRIMARY CULTURES OF AORTIC MEDIAL CELLS [J].

FISHERDZOGA, K ;

JONES, RM ;

VESSELINOVITCH, D ;

WISSLER, RW .

EXPERIMENTAL AND MOLECULAR PATHOLOGY, 1973, 18 (02) :162-+

[9] ARYL 4-MONO-OXYGENASE AND CYTOCHROME-P-450 IN AORTA - POSSIBLE ROLE IN ATHEROSCLEROSIS [J].

JUCHAU, MR ;

BOND, JA ;

BENDITT, EP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (10) :3723-3725

[10]

JUCHAU MR, 1979, POLYNUCLEAR AROMATIC, P639

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