USE OF STEADY-STATE RATE EQUATIONS TO ANALYZE PROGRESS CURVE DATA

被引：16

作者：

DUGGLEBY, RG ^{[1
]}

MORRISON, JF ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV, JOHN CURTIN SCH MED RES, DEPT BIOCHEM, CANBERRA 2601, ACT, AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 568卷 / 02期

关键词：

Progress curve analysis; Steady-state equation;

D O I：

10.1016/0005-2744(79)90303-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of progress curves for enzyme-catalyzed reactions was made by using a procedure that does not require the derivation of complex integrated rate equations. The method involves conversion of progress curve data to reaction velocities that are then fitted to the appropriate differential rate equation. Application of the procedure to data obtained for the reaction catalyzed by aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1) showed that the resulting values for the kinetic parameters agreed well with those obtained by conventional progress curve analysis.

引用

页码：357 / 362

页数：6

共 5 条

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BIZZOZERO, SA ;

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[2]

CLELAND WW, 1967, ADV ENZYMOL RAMB, V29, P1

[3] ANALYSIS OF PROGRESS CURVES FOR ENZYME-CATALYZED REACTIONS BY NONLINEAR-REGRESSION [J].

DUGGLEBY, RG ;

MORRISON, JF .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 481 (02) :297-312

[4] PROGRESS CURVE ANALYSIS IN ENZYME-KINETICS - MODEL DISCRIMINATION AND PARAMETER-ESTIMATION [J].

DUGGLEBY, RG ;

MORRISON, JF .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 526 (02) :398-409

[5] AN ALGORITHM FOR LEAST-SQUARES ESTIMATION OF NONLINEAR PARAMETERS [J].

MARQUARDT, DW .

JOURNAL OF THE SOCIETY FOR INDUSTRIAL AND APPLIED MATHEMATICS, 1963, 11 (02) :431-441

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