IMMUNODETECTION OF AFRICANIZED HONEY-BEE (HYMENOPTERA, APIDAE) SPECIFIC PROTEINS

被引：2

作者：

HUNG, ACF

机构：

[1] Bee Research Laboratory, Agricultural Research Service, USDA, Beltsville

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 1992年 / 20卷 / 05期

关键词：

APIS MELLIFERA; IMMUNOBLOTTING; POLYCLONAL ANTISERUM; SUBSPECIES;

D O I：

10.1016/0305-1978(92)90080-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four native honey bee proteins (AMP-1, -2, 3, -4) were detected on the IEF gel by the polyclonal antiserum at 1 : 1000 dilution in immunoblotting. AMP 1 and/or AMP-2 were found in all Africanized honey bees (AHB). AMP-3 was found in 88% of AHB. All European honey bees (EHB) had only AMP-4. However, AMP-4 was also found in 53% of AHB. The same antiserum detected three denatured proteins on the SDS-PAGE gel with M(r)s around 39,000, 42,000 and 60,000, All three were found in AHB and EHB.

引用

页码：395 / 399

页数：5