A MONOCLONAL ELISA FOR BOVINE VIRAL DIARRHEA PESTIVIRUS ANTIGEN-DETECTION IN PERSISTENTLY INFECTED CATTLE

被引：21
作者：
MIGNON, B
DUBUISSON, J
BARANOWSKI, E
KOROMYSLOV, I
ERNST, E
BOULANGER, D
WAXWEILER, S
PASTORET, PP
机构：
[1] Department of Virology-Immunology, Faculty of Veterinary Medicine, Institut de Chimie, Sart Tilman-Liége
来源：
JOURNAL OF VIROLOGICAL METHODS | 1991年 / 35卷 / 02期
关键词：
ELISA; BOVINE VIRAL DIARRHEA VIRUS; MONOCLONAL ANTIBODY;
D O I：
10.1016/0166-0934(91)90133-K
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field.
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页码：177 / 188
页数：12
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