REPLICATION OF FROG VIRUS-3 IN AN AMPHIBIAN CELL-LINE (XTC-2) DERIVED FROM XENOPUS-LAEVIS

被引：6

作者：

ELLIOTT, RM ^{[1
]}

ARNOLD, MK ^{[1
]}

KELLY, DC ^{[1
]}

机构：

[1] UNIV OXFORD, DEPT FORESTRY, OXFORD, ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 44卷 / JUL期

关键词：

D O I：

10.1099/0022-1317-44-1-89

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Frog virus 3 (FV3) replicated in a X. laevis cell line, XTC-2. The virus was titrated in XTC-2 cells by plaque assay, but the efficiency of plaquing is lower than in minnow (FHM) or hamster (BHK) cells. The rate of replication is greater in XTC-2 cells than in BHK cells and this was correlated with the appearance of virus specific polypeptides in infected cells. EM observations showed that FV3 replicates in the cytoplasm of XTC-2 cells and that the virus may leave the cell by budding at the plasma membrane.

引用

页码：89 / 97

页数：9

共 22 条

[1]

BINGENBRENDEL A, 1971, J MICROSC-PARIS, V11, P249

[2] ELECTRON-MICROSCOPIC OBSERVATION OF AFRICAN SWINE FEVER VIRUS DEVELOPMENT IN VERO CELLS [J].