Identification of hot spot residues at protein-protein interface

被引：30
|
作者：
Li, Lei [1 ]
Zhao, Bing [1 ]
Cui, Zhanhua [1 ]
Gan, Jacob [1 ]
Sakharkar, Meena Kishore [1 ]
Kangueane, Pandjassarame [1 ]
机构：
[1] Nanyang Technol Univ, Sch Mech & Aerosp Engn, Singapore 639798, Singapore
来源：
BIOINFORMATION | 2006年 / 1卷 / 04期
关键词：
protein-protein interaction; interface analysis; hot spot residues; inter-atomic interaction;
D O I：
10.6026/97320630001121
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
It is known that binding free energy of protein-protein interaction is mainly contributed by hot spot (high energy) interface residues. Here, we investigate the characteristics of hot spots by examining inter-atomic sidechain-sidechain interactions using a dataset of 296 alanine-mutated interface residues. Results show that hot spots participate in strong and energetically favorable sidechain-sidechain interactions. Subsequently, we describe a novel, yet simple 'hot spot' prediction model with an accuracy that is similar to many available approaches. The model is also shown to efficiently distinguish specific protein-protein interactions from non-specific interactions.
引用
收藏
页码：121 / +
页数：13
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