ANALYSIS OF FATTY-ACIDS FROM HUMAN LIPIDS BY GAS-CHROMATOGRAPHY

被引：62
|
作者：
HAAN, GJ [1 ]
VANDERHEIDE, S [1 ]
WOLTHERS, BG [1 ]
机构：
[1] STATE UNIV GRONINGEN HOSP, CENT LAB CLIN CHEM, GRONINGEN, NETHERLANDS
来源：
JOURNAL OF CHROMATOGRAPHY | 1979年 / 162卷 / 03期
关键词：
D O I：
10.1016/S0378-4347(00)81513-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid, quantitative method is described for the analysis of fatty acids from human lipids, namely serum lipids and lipids from adipose tissue biopsies. The method includes extraction of serum lipids with chloroform-methanol, hydrolysis with tetramethylammonium hydroxide, methylation with methyl iodide and N,N-dimethylformamide and gas chromatographic analysis on a Supelcoport SP-2330 column. Fat biopsies are analysed without extraction. Optimal hydrolysis conditions have been investigated. © 1979.
引用
收藏
页码：261 / 271
页数：11
相关论文
共 50 条
  • [1] GAS-CHROMATOGRAPHY OF FREE FATTY-ACIDS OF SKIN LIPIDS
    NAIDINA, VP
    AVETISYANTS, BL
    DUBININ, DM
    KOSMICHESKAYA BIOLOGIYA I AVIAKOSMICHESKAYA MEDITSINA, 1985, 19 (04): : 93 - 94
  • [2] GAS-CHROMATOGRAPHY OF FATTY-ACIDS
    SHANTHA, NC
    NAPOLITANO, GE
    JOURNAL OF CHROMATOGRAPHY, 1992, 624 (1-2): : 37 - 51
  • [3] MICRODETERMINATION OF FATTY-ACIDS BY GAS-CHROMATOGRAPHY
    KIMURA, T
    IWASE, H
    JAPAN ANALYST, 1973, 22 (07): : 872 - 875
  • [4] REACTION GAS-CHROMATOGRAPHY AND FATTY-ACIDS
    HARRIS, WE
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1975, 13 (11) : 514 - 515
  • [5] DIRECT ANALYSIS OF FREE FATTY-ACIDS IN BACTERIA BY GAS-CHROMATOGRAPHY
    BRONDZ, I
    OLSEN, I
    GEIBROEK, T
    JOURNAL OF CHROMATOGRAPHY, 1983, 274 (MAY): : 299 - 304
  • [6] QUANTITATION OF FATTY-ACIDS IN MILK BY GAS-CHROMATOGRAPHY
    WU, AB
    JUAN, WH
    CHENG, HW
    LIN, CW
    CHEN, CY
    JOURNAL OF FOOD AND DRUG ANALYSIS, 1995, 3 (03) : 163 - 172
  • [7] FATTY-ACIDS OF SERRATIA DETERMINED BY GAS-CHROMATOGRAPHY
    BERGAN, T
    GRIMONT, PAD
    GRIMONT, F
    CURRENT MICROBIOLOGY, 1983, 8 (01) : 7 - 11
  • [8] QUANTITATIVE-ANALYSIS OF FOOD FATTY-ACIDS BY CAPILLARY GAS-CHROMATOGRAPHY
    SLOVER, HT
    LANZA, E
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1979, 56 (12) : 933 - 943
  • [9] DETERMINATION OF LONG-CHAIN FATTY-ACIDS IN HUMAN TOTAL PLASMA-LIPIDS USING GAS-CHROMATOGRAPHY
    OZAWA, A
    TAKAYANAGI, K
    FUJITA, T
    HIRAI, A
    HAMAZAKI, T
    TERANO, T
    TAMURA, Y
    KUMAGAI, A
    BUNSEKI KAGAKU, 1982, 31 (02) : 87 - 91
  • [10] DETECTION OF TALLOW IN LARD BY GAS-CHROMATOGRAPHY OF FATTY-ACIDS
    JUAREZ, M
    MARTINEZCASTRO, I
    GRASAS Y ACEITES, 1981, 32 (02) : 93 - 96
12345