INDUCTION OF AROMATASE IN HUMAN GRANULOSA-CELLS BY BOTH FOLLICLE-STIMULATING-HORMONE AND INSULIN-LIKE GROWTH-FACTOR-I INVOLVES TYROSINE PHOSPHORYLATION

被引：6

作者：

COSTRICI, N ^{[1
]}

LUNENFELD, B ^{[1
]}

PARIENTE, C ^{[1
]}

DOR, J ^{[1
]}

RABINOVICI, J ^{[1
]}

KANETY, H ^{[1
]}

KARASIK, A ^{[1
]}

机构：

[1] CHAIM SHEBA MED CTR,INST ENDOCRINOL,IL-52621 TEL HASHOMER,ISRAEL

来源：

GYNECOLOGICAL ENDOCRINOLOGY | 1994年 / 8卷 / 03期

关键词：

GRANULOSA; AROMATASE; IGF-I; FSH; CAMP; TYROSINE PHOSPHORYLATION;

D O I：

10.3109/09513599409072453

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Evaluation of the intracellular signalling mechanisms of follicle stimulating hormone (FSH) and insulin-like growth factor-I (IGF-I) was performed in luteinized and non-luteinized human granulosa cells. A severalfold increase in estradiol production from androstenedione was induced by both hormones in these cells, while only FSH led to a concomitant increase in cAMP. IGF-I bound specifically to its receptor in these cells. Specific tyrosine kinase inhibitors (tyrphostins) blocked the effects of both FSH and IGF-I on aromatase activity without altering FSH-induced cAMP accumulation. These findings demonstrate an involvement of a tyrosine kinase pathway in the intracellular signalling mechanism of the IGF-I effect on aromatase activity. Furthermore, since FSH induction of aromatase activity can be blocked by a tyrosine kinase inhibitor without affecting the level of cAMP production, it can be suggested that tyrosine kinase(s) act downstream of cAMP production and protein kinase A activation.

引用

页码：183 / 189

页数：7

共 25 条

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