SEPARATION OF MONOCLONAL-ANTIBODIES FROM ANTIHAPTEN ANTISERA BY 2-DIMENSIONAL AFFINITY ELECTROPHORESIS

被引：8

作者：

TAKEO, K

NAKAMURA, K

SUZUNO, R

机构：

[1] The First Department of Biochemistry, Yamaguchi University School of Medicine, Ube, 755

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80133-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-resolution two-dimensional affinity electrophoresis (2D-AEP) method was developed, using capillary polyacrylamide gel (PAG) isoelectric focusing in the first and slab PAG affinity electrophoresis in the second direction. Using this method, anti-hapten antibodies were separated into a number of monoclonal antibody [immunoglobulin G (IgG)] families, each of which is composed of several IgG spots having an identical affinity to the hapten but different isoelectric points. 2D-AEP may offer a powerful tool for solving fundamental problems in immunochemistry such as antibody heterogeneity, its happen binding specificity and antigen-dependent somatic mutation.

引用

页码：365 / 376

页数：12

共 11 条

[1]

BOGHANSEN TC, 1980, J ELECTROPHORESIS, V1, P67

[2]

EISEN H N, 1964, Methods Med Res, V10, P94

[3] FLUORESCENT PROBES FOR STUDY OF ANTIBODY-HAPTEN REACTION .I. BINDING OF 5-DIMETHYLAMINONAPHTHALENE-1-SULFONAMIDO GROUP BY HOMOLOGOUS RABBIT ANTIBODY [J].