ISOLATION AND DETERMINATION OF SUGARS IN NICOTIANA-TABACUM ON AMINOPROPYL CHEMICALLY BONDED PHASE USING SPE AND HPLC

Aminopropyl chemically bonded phases for solid-phase extraction (SPE) and high performance liquid chromatography (HPLC) have been prepared. Surface characteristics of packings before and after chemical modification were determined by means of different physico-chemical methods e.g. porosimetry, elemental analysis, CP/MAS NMR, chromatography, etc. The obtained packing materials were compared with commercially available packings (Silasorb NH2 and LiChrosorb NH2). Prepared columns were applied for the isolation and determination of sugars origined from dried leaves of the Nicotiana tabacum. The columns were characterized by high coverage density of aminopropyl phase and high sorption capacity gave good and reproducible separation results.