PREPARATION AND STABILITY OF EXO-AMYLOLYTIC ENZYMES CHEMICALLY COUPLED TO MICROCRYSTALLINE CELLULOSE

被引：46

作者：

BARKER, SA

SOMERS, PJ

EPTON, R

机构：

[1] Department of Chemistry, The University

[2] Department of Applied Science, Wolverhampton College of Technology, Wolverhampton

来源：

CARBOHYDRATE RESEARCH | 1969年 / 9卷 / 03期

关键词：

D O I：

10.1016/S0008-6215(00)80163-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of active, water-insoluble derivatives of β- and γ-amylase by chemical coupling with a diazotised 3-(p-aminophenoxy)-2-hydroxypropyl ether of cellulose, and a water-insoluble derivative of β-amylase by coupling with a 2-hydroxy-3-(p-isothiocyanatophenoxy)propyl ether of cellulose is described. The activity retained on insolubilisation is considerably higher than that commonly retained on water-insolubilisation of hydrolytic enzymes active against macromolecular substrates. It is suggested that this is connected with the exo-hydrolytic character of β- and γ-amylase. The insoluble enzymes are relatively more resistant to heat denaturation than the free enzymes in solution. © 1969.

引用

页码：257 / &

共 10 条

[1] CHEMICAL COUPLING OF ENZYMES TO CROSS-LINKED DEXTRAN (SEPHADEX) [J].