CREATING A CONDITIONAL MUTATION OF WNT-1 BY ANTISENSE TRANSGENESIS PROVIDES EVIDENCE THAT WNT-1 IS NOT ESSENTIAL FOR SPERMATOGENESIS

被引:16
|
作者
ERICKSON, RP
LAI, LW
GRIMES, J
机构
[1] UNIV ARIZONA,COLL MED,DEPT PEDIAT,TUCSON,AZ 85724
[2] UNIV ARIZONA,COLL MED,DEPT MOLEC & CELLULAR BIOL,TUCSON,AZ 85724
来源
DEVELOPMENTAL GENETICS | 1993年 / 14卷 / 04期
关键词
TRANSGENESIS; ANTISENSE RNA; WINGLESS; SPERMATOGENESIS; PHOSPHOGLYCERATE KINASE-2 PROMOTER;
D O I
10.1002/dvg.1020140405
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have used mice transgenic for an antisense construct for Wnt-1 to study the role of this gene in post-meiotic sperm development. The human PGK-2 promoter provided levels of Wnt-1 antisense mRNA in testes in 5 transgenic lines greatly in excess of Wnt-1 mRNA concentrations, and Wnt-1 mRNA levels were greatly decreased in the lines, by 98% in three of them. There was a general correlation between copy number of the insert, levels of antisense RNA, and decreases in mRNA. There was little effect of the antisense transgene on fertility or testicular histology suggesting that normal levels of Wnt-1 transcript are not essential for spermatogenesis. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码:274 / 281
页数:8
相关论文
共 50 条
  • [1] INTERACTIONS OF WNT-1 AND WNT-3A ARE ESSENTIAL FOR NEURAL-TUBE PATTERNING
    AUGUSTINE, KA
    LIU, ET
    SADLER, TW
    TERATOLOGY, 1995, 51 (02) : 107 - 119
  • [2] A secreted Frizzled related protein, FrzA, selectively associates with Wnt-1 protein and regulates Wnt-1 signaling
    Dennis, S
    Aikawa, M
    Szeto, W
    d'Amore, PA
    Papkoff, J
    JOURNAL OF CELL SCIENCE, 1999, 112 (21) : 3815 - 3820
  • [3] ACTIVITY OF WNT-1 AS A TRANSMEMBRANE PROTEIN
    PARKIN, NT
    KITAJEWSKI, J
    VARMUS, HE
    GENES & DEVELOPMENT, 1993, 7 (11) : 2181 - 2193
  • [4] The induction of ret by Wnt-1 in PC12 cells is atypically dependent on continual Wnt-1 expression
    Zheng, S
    Ramachandran, B
    Haigh, JR
    Palos, TP
    Steger, K
    Howard, BD
    ONCOGENE, 1996, 12 (03) : 555 - 562
  • [5] WNT-1 AND WNT-2 ARE POTENT INDUCERS OF NEPHROGENESIS
    HERZLINGER, D
    QIAO, JZ
    COHEN, D
    RAMAKRISHNA, N
    BROWN, AMC
    FASEB JOURNAL, 1994, 8 (05): : A822 - A822
  • [6] Wnt-1 and Wnt-4 regulate thymic cellularity
    Mulroy, T
    McMahon, JA
    Burakoff, SJ
    McMahon, AP
    Sen, J
    EUROPEAN JOURNAL OF IMMUNOLOGY, 2002, 32 (04) : 967 - 971
  • [7] SWAYING IS A MUTANT ALLELE OF THE PROTOONCOGENE WNT-1
    THOMAS, KR
    MUSCI, TS
    NEUMANN, PE
    CAPECCHI, MR
    CELL, 1991, 67 (05) : 969 - 976
  • [8] Increased expression of Wnt-1 in schizophrenic brains
    Miyaoka, T
    Seno, H
    Ishino, H
    SCHIZOPHRENIA RESEARCH, 1999, 38 (01) : 1 - 6
  • [9] Regulation of dorsal fate in the neuraxis by Wnt-1 and Wnt-3a
    Saint-Jeannet, J.-P.
    He, X.
    Varmus, H. E.
    Dawid, I. B.
    Proceedings of the National Academy of Sciences of the United States of America, 94 (25):
  • [10] THE WNT-1 GENE FAMILY REGULATORS OF DEVELOPMENT
    MCMAHON, AP
    BRADLEY, A
    GAVIN, B
    PARR, B
    MCMAHON, JA
    FASEB JOURNAL, 1991, 5 (04): : A783 - A783