DIFFERENTIATION OF SALMONELLA PHASE-1 FLAGELLAR ANTIGEN TYPES BY RESTRICTION OF THE AMPLIFIED FLIC GENE

被引:53
作者
KILGER, G [1 ]
GRIMONT, PAD [1 ]
机构
[1] INST PASTEUR,INSERM,UNITE 199,UNITE ENTEROBACTERIES,F-75724 PARIS 15,FRANCE
关键词
D O I
10.1128/JCM.31.5.1108-1110.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The large antigenic diversity (over 2,300 serotypes) expressed by Salmonella strains can probably be observed at the genetic level. The phase 1 flagellin gene fliC was amplified, and the amplified fragment was cleaved with a mixture of both endonucleases TaqI and ScaI. The restriction patterns observed allowed differentiation of flagellar types b, i, d, j, l, v, and z10. Flagellar group g (g,m, g, p, or g, m, s) could be differentiated from the other flagellar types. Flagellar types r and e,h could not be separated, although they could be distinguished from the other flagellar types studied. Practical applications of flagellar gene restriction are the distinction between serotype Gallinarum-Pullorum, which carries a cryptic gene for flagellar type g, m, and nonmotile Vi-negative variants of serotype Typhi, and the tentative assignation of nonmotile variants of Salmonella serotypes to a flagellar type.
引用
收藏
页码:1108 / 1110
页数:3
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