HIGH-EFFICIENCY FORMATION OF INFLUENZA-VIRUS TRANSFECTANTS

被引：111

作者：

ENAMI, M

PALESE, P

机构：

[1] CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA

[2] NATL INST HLTH, DEPT MEASLES VIRUS, Musashimurayama, TOKYO 19012, JAPAN

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 05期

关键词：

D O I：

10.1128/JVI.65.5.2711-2713.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.

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页码：2711 / 2713

页数：3

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