PURIFICATION AND CHARACTERIZATION OF A PROTEIN FACTOR THAT REVERSES THE INHIBITION OF PROTEIN-SYNTHESIS BY THE HEME-REGULATED TRANSLATIONAL INHIBITOR IN RABBIT RETICULOCYTE LYSATES

被引：97

作者：

AMESZ, H

GOUMANS, H

HAUBRICHMORREE, T

VOORMA, HO

BENNE, R

机构：

[1] Vakgroep Moleculaire Celbiologie, Rijksuniversiteit te Utrecht, Universiteit's Centrum ‘De Uithof’, Utrecht, 3584 CH

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13212.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified and partially characterized a supernatant factor which reverses the effect of the heme‐regulated translational inhibitor on protein synthesis in rabbit reticulocyte lysates. The anti‐inhibitor restores protein synthesis activity in heme‐deficient lysates (and in lysates to which the inhibitor has been added) to the level observed in the presence of heme. The factor has no effect on the phosphorylation of eIF‐2 by the inhibitor nor on any reaction carried out with purified initiation factors. The anti‐inhibitor probably consists of three subunits with molecular weights of 81000, 60000 and 41000. The factor is isolated from the postribosomal supernatant of rabbit reticulocytes both free and complexed to eIF‐2. A possible mechanism of action is discussed. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：513 / 520

页数：8

共 50 条

[1] REGULATION OF PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTE LYSATES - PURIFICATION AND CHARACTERIZATION OF HEME-REVERSIBLE TRANSLATIONAL INHIBITOR
TRACHSEL, H
RANU, RS
LONDON, IM
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) : 3654 - 3658
[2] REGULATION OF PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTE LYSATES - PURIFICATION AND INITIAL CHARACTERIZATION OF CYCLIC 3'-5'-AMP INDEPENDENT PROTEIN-KINASE OF HEME-REGULATED TRANSLATIONAL INHIBITOR (PHOSPHORYLATION OF MET-TRNAF BINDING FACTOR)
RANU, RS
LONDON, IM
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (12) : 4349 - 4353
[3] PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTES - MECHANISM OF PROTEIN-SYNTHESIS INHIBITION BY HEME-REGULATED INHIBITOR .24.
DAS, A
RALSTON, RO
GRACE, M
ROY, R
GHOSHDASTIDAR, P
DAS, HK
YAGHMAI, B
PALMIERI, S
GUPTA, NK
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (10) : 5076 - 5079
[4] PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTES - CHARACTERISTICS OF THE PROTEIN FACTOR RF THAT REVERSES INHIBITION OF PROTEIN-SYNTHESIS IN HEME-DEFICIENT RETICULOCYTE LYSATES .30.
GRACE, M
RALSTON, RO
BANERJEE, AC
GUPTA, NK
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21): : 6517 - 6521
[5] REGULATION OF PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTE LYSATES - THIOPHOSPHORYLATION OF INITIATION-FACTOR EIF-2 BY HEME-REGULATED PROTEIN-KINASE
RANU, RS
FEBS LETTERS, 1986, 208 (01) : 117 - 122
[6] PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTES - FURTHER-STUDIES OF THE CHARACTERISTICS OF THE PROTEIN FACTOR (RF) THAT REVERSES THE INHIBITION OF PROTEIN-SYNTHESIS IN HEME-DEFICIENT RETICULOCYTE LYSATES
RALSTON, RO
GRACE, M
DAS, A
GUPTA, NK
FEDERATION PROCEEDINGS, 1981, 40 (06) : 1549 - 1549
[7] PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTES - A STUDY OF THE MECHANISM OF ACTION OF THE PROTEIN FACTOR-RF THAT REVERSES PROTEIN-SYNTHESIS INHIBITION IN HEME-DEFICIENT RETICULOCYTE LYSATES
GRACE, M
BAGCHI, M
AHMAD, F
YEAGER, T
OLSON, C
CHAKRAVARTY, I
NASRIN, N
BANERJEE, A
GUPTA, NK
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (17): : 5379 - 5383
[8] MOLECULAR MECHANISM OF INHIBITION OF PROTEIN-SYNTHESIS BY HEME-REGULATED INHIBITOR FROM RABBIT RETICULOCYTES
RANU, RS
DAS, A
DASGUPTA, A
MAJUMDER, A
RALSTON, R
ROY, R
GUPTA, NK
LONDON, IM
BLOOD, 1977, 50 (05) : 117 - 117
[9] MOLECULAR MECHANISM OF INHIBITION OF PROTEIN-SYNTHESIS BY A PARTIALLY PURIFIED TRANSLATIONAL INHIBITOR FROM RABBIT RETICULOCYTE LYSATES
RANU, R
LEVIN, D
CLEMENS, M
CHERBAS, L
LONDON, IM
FEDERATION PROCEEDINGS, 1975, 34 (03) : 621 - 621
[10] PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTES - CHARACTERISTICS OF A POSTRIBOSOMAL SUPERNATANT FACTOR THAT REVERSES INHIBITION OF PROTEIN-SYNTHESIS IN HEME-DEFICIENT LYSATES AND INHIBITION OF TERNARY COMPLEX (MET-TRANSFERRNAFMET.EIF-2.GTP) FORMATION BY HEME-REGULATED INHIBITOR .25.
RALSTON, RO
DAS, A
GRACE, M
DAS, H
GUPTA, NK
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (11) : 5490 - 5494

← 1 2 3 4 5 →