HIGH-LEVEL EXPRESSION AND SECRETION OF STREPTOKINASE IN ESCHERICHIA-COLI

被引：36

作者：

KO, JH ^{[1
]}

PARK, DK ^{[1
]}

KIM, IC ^{[1
]}

LEE, SH ^{[1
]}

BYUN, SM ^{[1
]}

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT BIOL SCI,YUSUNG KU,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 10期

关键词：

D O I：

10.1007/BF00143093

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 mi of LB-ampicillin media. About 95% of the expressed streptokinase was secreted into the periplasmic and extracellular fractions. The recombinant streptokinase in high yield and purity may be a potential alternative source for the therapeutic agent.

引用

页码：1019 / 1024

页数：6

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