ENZYME-AIDED CONSTRUCTION OF MEDIUM-SIZED ALDITOLS OF COMPLETE O-LINKED SACCHARIDES - THE CONSTRUCTED HEXASACCHARIDE ALDITOL GAL-BETA-1-4GLCNAC-BETA-1-6GAL-BETA-1-4GLCNAC-BETA-1-6(GAL-BETA-1-3)GALNAC-OL RESISTS THE ACTION OF ENDO-BETA-GALACTOSIDASE FROM BACTEROIDES-FRAGILIS

被引：9

作者：

MAAHEIMO, H

PENTTILA, L

RENKONEN, O

机构：

[1] UNIV HELSINKI,INST BIOTECHNOL,SF-00014 HELSINKI,FINLAND

[2] UNIV HELSINKI,DEPT BIOCHEM,SF-00014 HELSINKI,FINLAND

来源：

FEBS LETTERS | 1994年 / 349卷 / 01期

关键词：

ENDO-BETA-GALACTOSIDASE; O-LINKED SACCHARIDE; GLCNAC-BETA-1-6GAL; IN VITRO SYNTHESIS; NMR; HPAEC;

D O I：

10.1016/0014-5793(94)00638-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed by enzyme-aided in vitro synthesis a hexasaccharide alditol Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol and shown that it resists the action of endo-beta-galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo-beta-galactosidase from B. fragilis, apparently, can be used to distinguish between GlcNAc beta 1-6Gal and GlcNAc beta 1-3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans.

引用

页码：55 / 59

页数：5

共 5 条

[1] SINGLE MIDCHAIN GLCNAC-BETA-1-6GAL-BETA-1-4R SEQUENCES OF LINEAR OLIGOSACCHARIDES ARE RESISTANT TO ENDO-BETA-GALACTOSIDASE OF BACTEROIDES-FRAGILIS
RENKONEN, O
PENTTILA, L
NIEMELA, R
LEPPANEN, A
GLYCOCONJUGATE JOURNAL, 1991, 8 (04) : 376 - 380
[2] ENZYMATIC INVITRO SYNTHESIS OF RADIOLABELED PENTASACCHARIDES GLCNAC-BETA-1-3(GAL-BETA-1-4GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC/GLC AND THE ISOMERIC GAL-BETA-1-4GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC/GLC
RENKONEN, O
LEPPANEN, A
NIEMELA, R
VILKMAN, A
HELIN, J
PENTTILA, L
MAAHEIMO, H
SEPPO, A
SUOPANKI, J
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1992, 70 (01): : 86 - 89
[3] A NOVEL BETA-N-ACETYLGLUCOSAMINIDASE ACTIVITY IN HOG GASTRIC-MUCOSAL MICROSOMES - PREFERENTIAL HYDROLYSIS OF TERMINAL GLCNAC-BETA-1-3 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC, BUT GLCNAC-BETA-1-6 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL
HELIN, J
SEPPO, A
LEPPANEN, A
PENTTILA, L
MAAHEIMO, H
NIEMELA, R
LAURI, S
RENKONEN, O
FEBS LETTERS, 1993, 335 (02) : 280 - 284
[4] H-1 and C-13 NMR analysis of the pentasaccharide Gal beta(1->4)GlcNAc beta(1->3)-[GlcNAc beta(1->6)]Gal beta(1->4)GlcNAc synthesized by the mid-chain beta-(1->6)-D-N-acetylglucosaminyltransferase of rat serum
Maaheimo, H
Rabina, J
Renkonen, O
CARBOHYDRATE RESEARCH, 1997, 297 (02) : 145 - 151
[5] HOMONUCLEAR 3-DIMENSIONAL NMR METHODS FOR THE COMPLETE ASSIGNMENT OF PROTON NMR-SPECTRA OF OLIGOSACCHARIDES - APPLICATION TO GAL-BETA-1-4(FUC-ALPHA-1-3)GLCNAC-BETA-1-3GAL-BETA-1-4GLC
HOMANS, SW
GLYCOBIOLOGY, 1992, 2 (02) : 153 - 159

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