ENZYME-AIDED CONSTRUCTION OF MEDIUM-SIZED ALDITOLS OF COMPLETE O-LINKED SACCHARIDES - THE CONSTRUCTED HEXASACCHARIDE ALDITOL GAL-BETA-1-4GLCNAC-BETA-1-6GAL-BETA-1-4GLCNAC-BETA-1-6(GAL-BETA-1-3)GALNAC-OL RESISTS THE ACTION OF ENDO-BETA-GALACTOSIDASE FROM BACTEROIDES-FRAGILIS

被引：9
作者：
MAAHEIMO, H
PENTTILA, L
RENKONEN, O
机构：
[1] UNIV HELSINKI,INST BIOTECHNOL,SF-00014 HELSINKI,FINLAND
[2] UNIV HELSINKI,DEPT BIOCHEM,SF-00014 HELSINKI,FINLAND
来源：
FEBS LETTERS | 1994年 / 349卷 / 01期
关键词：
ENDO-BETA-GALACTOSIDASE; O-LINKED SACCHARIDE; GLCNAC-BETA-1-6GAL; IN VITRO SYNTHESIS; NMR; HPAEC;
D O I：
10.1016/0014-5793(94)00638-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have constructed by enzyme-aided in vitro synthesis a hexasaccharide alditol Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol and shown that it resists the action of endo-beta-galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo-beta-galactosidase from B. fragilis, apparently, can be used to distinguish between GlcNAc beta 1-6Gal and GlcNAc beta 1-3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans.
引用
收藏
页码：55 / 59
页数：5
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