RADIOLYTIC SIGNATURE OF Z-DNA

被引:42
作者
TARTIER, L
MICHALIK, V
SPOTHEIMMAURIZOT, M
RAHMOUNI, AR
SABATTIER, R
CHARLIER, M
机构
[1] CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 2,FRANCE
[2] CHR HOSP ORLEANS,SERV RADIOTHERAPIE,F-45067 ORLEANS 2,FRANCE
关键词
D O I
10.1093/nar/22.25.5565
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ionizing radiations induce various damages in DNA via the hydroxyl radical OH. generated by the radiolysis of water. We compare here the radiosensitivity of BE and Z-DNA, by using a Z-prone stretch included in a plasmid. In the supercoiled plasmid, the stretch is in the Z-form, whereas it is in the B-form when the plasmid is relaxed. Frank strand breaks (FSB) and alkali-revealed breaks (ARE) were located and quantified using sequencing gel electrophoresis. We show that B- and Z-DNA have the same mean sensitivity towards radiolytic attack, for both FSB and ARE. Nevertheless, the guanine sites are more sensitive, and the cytosine sites less sensitive in Z- than in B-DNA, leading to a characteristic signature of the Z-form. The comparison of experiments with the outcome of a Monte Carlo simulation of OH. radical attack suggests that transfer of initial damage from a guanine base to its attached sugar or the adjacent 3' cytosine is more important in Z-DNA than in B-DNA.
引用
收藏
页码:5565 / 5570
页数:6
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