ANALYSIS OF DISULFIDE-CONTAINING FRAGMENTS OF NA+, K+-ATPASE .1. QUANTITATION OF DISULFIDE BONDS OF THE ALPHA-SUBUNIT

被引：0
作者：
GEVONDYAN, NM [1 ]
GAVRILYEVA, EE [1 ]
GEVONDYAN, VS [1 ]
MODYANOV, NN [1 ]
机构：
[1] KOVALENKO RES INST EXPTL VET,MOSCOW,RUSSIA
来源：
BIOLOGICHESKIE MEMBRANY | 1993年 / 10卷 / 06期
关键词：
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Ammetric titration with silver nitrate revealed the presence in pig kidney Na+, K+-ATPase of five disulfide bonds and twenty free cysteine residues, most of which are masked. Complete alkylation of all of free SH groups was found possible only after preliminary digestion of the membrane-bound Na+, K+-ATPase. A fraction of disulfide-containing peptides involving three fragments of the alpha-subunit polypeptide chain, namely: Cys(452)-Lys(461), Ile(507)-Lys(519) Val(545)-Phe(558), has been isolated from the tryptic digest alkylated with 4-vinylpyridine. Reduction of SS bonds with beta-mercaptoethanol and alkylation of the released cysteine residues with radiolabeled iodoacetic acid indicated that three above fragments contained cysteine residues that are involved in the formation of two disulfide bonds.
引用
收藏
页码：632 / 640
页数：9
相关论文
共 50 条
[41]   Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors [J].
María Geraldina Bersier ;
Clara Peña ;
Georgina Rodríguez de Lores Arnaiz .
Neurochemical Research, 2011, 36 :297-303
[42]   A NEW ELECTROPHORETIC VARIANT OF ALPHA-SUBUNIT OF NA+/K+-ATPASE FROM THE SUBMANDIBULAR-GLAND OF RATS [J].
KURIHARA, K ;
HOSOI, K ;
KODAMA, A ;
UEHA, T .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1039 (02) :234-240
[43]   Regulation of Na+,K+-ATPase alpha-subunit expression by mechanical strain in aortic smooth muscle cells [J].
SonguMize, E ;
Liu, X ;
Stones, JE ;
Hymel, LJ .
HYPERTENSION, 1996, 27 (03) :827-832
[44]   cDNA cloning of Na+,K+-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus [J].
MitsunagaNakatsubo, K ;
Yamazaki, K ;
HatohOkazaki, M ;
Kawashita, H ;
Okamura, C ;
Akasaka, K ;
Shimada, H ;
Yasumasu, I .
ZOOLOGICAL SCIENCE, 1996, 13 (06) :833-841
[45]   CHROMOSOMAL LOCALIZATION OF THE GENE FOR THE ALPHA-SUBUNIT OF NA+, K+-ATPASE (AATP) IN THE AMERICAN MINK MUSTELA VISION [J].
MATVEEVA, NM ;
KHLEBODAROVA, TM ;
KARASIK, GI ;
RUBTSOV, NV ;
SEROV, OL ;
SVERDLOV, ED ;
BROUDE, NE ;
MODIANOV, NN ;
MONASTYRSKAIA, GS ;
OVCHINNIKOV, IA .
DOKLADY AKADEMII NAUK SSSR, 1987, 294 (04) :998-1000
[46]   ISOFORM-SPECIFIC MODULATION OF NA+,K+-ATPASE ALPHA-SUBUNIT GENE-EXPRESSION IN HYPERTENSION [J].
HERRERA, VLM ;
CHOBANIAN, AV ;
RUIZOPAZO, N .
SCIENCE, 1988, 241 (4862) :221-223
[47]   QUANTITATIVE IMMUNOGOLD LOCALIZATION OF NA+,K+-ATPASE ALPHA-SUBUNIT IN THE TYMPANIC WALL OF RAT COCHLEAR DUCT [J].
IWANO, T ;
YAMAMOTO, A ;
OMORI, K ;
KAWASAKI, K ;
KUMAZAWA, T ;
TASHIRO, Y .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (02) :225-232
[48]   THE FAMILY OF HUMAN NA+,K+-ATPASE GENES - A PARTIAL NUCLEOTIDE-SEQUENCE RELATED TO THE ALPHA-SUBUNIT [J].
OVCHINNIKOV, YA ;
MONASTYRSKAYA, GS ;
BROUDE, NE ;
ALLIKMETS, RL ;
USHKARYOV, YA ;
MELKOV, AM ;
SMIRNOV, YV ;
MALYSHEV, IV ;
DULUBOVA, IE ;
PETRUKHIN, KE ;
GRYSHIN, AV ;
SVERDLOV, VE ;
KIYATKIN, NI ;
KOSTINA, MB ;
MODYANOV, NN ;
SVERDLOV, ED .
FEBS LETTERS, 1987, 213 (01) :73-80
[49]   QUANTITATIVE IMMUNOCYTOCHEMICAL LOCALIZATION OF NA+,K+-ATPASE ALPHA-SUBUNIT IN THE LATERAL WALL OF RAT COCHLEAR DUCT [J].
IWANO, T ;
YAMAMOTO, A ;
OMORI, K ;
AKAYAMA, M ;
KUMAZAWA, T ;
TASHIRO, Y .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1989, 37 (03) :353-363
[50]   REGULATION OF NA+,K+-ATPASE ALPHA-SUBUNIT GENE-EXPRESSION DURING MOUSE PREIMPLANTATION DEVELOPMENT [J].
MACPHEE, DJ ;
BARR, KJ ;
DESOUSA, PA ;
TODD, SDL ;
KIDDER, GM .
DEVELOPMENTAL BIOLOGY, 1994, 162 (01) :259-266
12345