ANALYSIS OF POTATO MICROTUBER PROTEINS BY SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS

Protein banding patterns of 119 lines (85 Japanese potato lines and introductions, 22 dihaploids, 1 wild source (Solanum bulbocastanum Dun.), 10 transformants and 1 somaclonal variant) were compared by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Microtubers formed in vitro were used as the source of plant materials. There were more than 15 bands in the 35-20 kDa molecular weight range and less than 10 bands in the 65-40 kDa range. The bands in the 65-40 kDa range which corresponded to the potato tuber storage protein patatin were focussed to about 40 kDa after heat treatment. The results indicate a distinct difference in the banding patterns among potato lines.