RAPID SURVEYING OF DNA-SEQUENCE VARIATION IN NATURAL-POPULATIONS

被引：0

作者：

LESSA, EP

机构：

来源：

MOLECULAR BIOLOGY AND EVOLUTION | 1992年 / 9卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; DENATURING GRADIENT GEL ELECTROPHORESIS; INTRON; BETA-GLOBIN;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequencing can be costly and time consuming for population studies because of the relative rarity of variation along exons. These problems can be substantially reduced by the use of the polymerase chain reaction on introns using primers from the exon region. These problems can be further reduced by the use of denaturing gradient gel electrophoresis to identify those alleles in need of sequencing.

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页码：323 / 330

页数：8

相关论文

共 21 条

[1] COMPREHENSIVE DETECTION OF SINGLE BASE CHANGES IN HUMAN GENOMIC DNA USING DENATURING GRADIENT GEL-ELECTROPHORESIS AND A GC CLAMP [J].

ABRAMS, ES ;

MURDAUGH, SE ;

LERMAN, LS .

GENOMICS, 1990, 7 (04) :463-475

[2] GENE TREES AND ORGANISMAL HISTORIES - A PHYLOGENETIC APPROACH TO POPULATION BIOLOGY [J].

AVISE, JC .

EVOLUTION, 1989, 43 (06) :1192-1208

[3] GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS [J].

GYLLENSTEN, UB ;

ERLICH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7652-7656

[4] DYNAMICS OF MITOCHONDRIAL-DNA EVOLUTION IN ANIMALS - AMPLIFICATION AND SEQUENCING WITH CONSERVED PRIMERS [J].

KOCHER, TD ;

THOMAS, WK ;

MEYER, A ;

EDWARDS, SV ;

PAABO, S ;

VILLABLANCA, FX ;

WILSON, AC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6196-6200

[5] EFFECTS OF PRIMER TEMPLATE MISMATCHES ON THE POLYMERASE CHAIN-REACTION - HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MODEL STUDIES [J].

KWOK, S ;

KELLOGG, DE ;

MCKINNEY, N ;

SPASIC, D ;

GODA, L ;

LEVENSON, C ;

SNINSKY, JJ .

NUCLEIC ACIDS RESEARCH, 1990, 18 (04) :999-1005

[6]

Maniatis T., 1982, MOL CLONING

[7]

Miller S. A., 1998, NUCLEIC ACIDS RES, V16, P215, DOI DOI 10.1093/NAR/16.3.1215

[8]

Myers R, 1989, PCR TECHNOLOGY PRINC, P71

[9]

MYERS RM, 1987, METHOD ENZYMOL, V155, P501

[10]

MYERS RM, 1989, MUTATION DETECTION P, P71