PREVALENCE OF HUMAN PAPILLOMAVIRUS TYPE-16 AND TYPE-18 IN CERVICAL CARCINOMAS - A STUDY BY DOT AND SOUTHERN BLOT HYBRIDIZATION AND THE POLYMERASE CHAIN-REACTION

被引：18
作者：
LOW, SH
THONG, TW
HO, TH
LEE, YS
MORITA, T
SINGH, M
YAP, EH
CHAN, YC
机构：
[1] NATL UNIV SINGAPORE, DEPT MICROBIOL, LOWER KENT RIDGE RD, SINGAPORE 0511, SINGAPORE
[2] NATL UNIV SINGAPORE, SINGAPORE GEN HOSP, DEPT OBST & GYNAECOL, SINGAPORE 0511, SINGAPORE
[3] NATL UNIV SINGAPORE, DEPT PATHOL, SINGAPORE 0511, SINGAPORE
[4] OSAKA UNIV, MICROBIAL DIS RES INST, DEPT MICROBIAL GENET, SUITA, OSAKA 565, JAPAN
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1990年 / 81卷 / 11期
关键词：
Cervical cancer; Dot blot; Human papillomavirus (HPV) 16 and 18; Polymerase chain reaction; Southern blot;
D O I：
10.1111/j.1349-7006.1990.tb02522.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (HPV) types 16 and 18 infection. Sixty of the 83 (72.3%) were found to contain HPV DNA, of which 43 (51.8%) contained HPV 16 DNA, 12 (14.5%) contained HPV 18 DNA and 5 (6.0%) contained both HPV 16 and 18 DNAs. Southern blot analysis on 65 specimens gave similar results. Of 23 specimens negative by dot blot, 21 were tested by the polymerase chain reaction. Seventeen of the 21 were positive for HPV DNA, of which 13 contained HPV 16 DNA and 4 contained both HPV 16 and 18 DNAs. In all, 95.1% (77/81) were positive for HPV 16 and/or 18 DNA sequences. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码：1118 / 1123
页数：6
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