ERYTHROCYTE NA-K-CL COTRANSPORT ACTIVITY IN LOW RENIN ESSENTIAL HYPERTENSIVE PATIENTS - A NA-23 NUCLEAR-MAGNETIC-RESONANCE STUDY

被引：6

作者：

CACCIAFESTA, M ^{[1
]}

FERRI, C ^{[1
]}

CARLOMAGNO, A ^{[1
]}

DEANGELIS, C ^{[1
]}

SCUTERI, A ^{[1
]}

GUIDONI, L ^{[1
]}

LUCIANI, AM ^{[1
]}

ROSI, A ^{[1
]}

VITI, V ^{[1
]}

SANTUCCI, A ^{[1
]}

BALSANO, F ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,FDN ANDREA CESALPINO,INST CLIN MED 1,I-00161 ROME,ITALY

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1994年 / 7卷 / 02期

关键词：

SODIUM; POTASSIUM; CELL MEMBRANE PERMEABILITY; RENIN-ANGIOTENSIN SYSTEM; HYPERTENSION;

D O I：

10.1093/ajh/7.2.151

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

The Na-K-Cl cotransport activity in red blood cells from essential hypertensive men with low (n = 8, mean age 42 +/- 4 years) or normal renin activity (n = 4, mean age 43 +/- 3 years), and in normotensive men with normal renin activity (n = 7, mean age 38 +/- 4 years) has been evaluated by means of a recently developed Na-23 nuclear magnetic resonance (NMR) method. Sodium efflux was determined by relating the resonating frequency of the NMR signal from extracellular sodium to sodium concentration in the presence of the shift reagent Dy(PPP)(2)(7-). The maximum Na+ efflux driven by cotransport (V-max) was measured in Na+-loaded erythrocytes in the presence of ouabain to block the Na-K-Cl pump activity. A significant difference (P < 0.05) was found in V-max values of low renin patients (0.70 mmol/h/L cells, range 0.40 to 0.90 mmol/h/L cells) as compared with normotensive controls (0.39 +/- 0.08 mmol/h/L cells) and normal renin hypertensives (mean 0.49 +/- 0.04 mmol/h/L cells). In conclusion, this study showed an increased activity of the Na-K-CI cotransport in red blood cells from low renin hypertensive men as compared with normal renin hypertensives and normotensives.

引用

页码：151 / 158

页数：8

共 37 条

[1] Cusi D., Barlassina D., Tripodi G., Bianchi G., Genetic factors involved in ion transport abnormalities in essential hypertension, J Hypertens, 5, pp. S255-S259, (1987)
[2] Ferrari P., Ferrandi M., Torielli M., Et al., Relationship between erythrocyte volume and sodium transport in the Milan hypertensive rat and age dependent changes, J Hypertens, 5, pp. 199-206, (1987)
[3] De Mendonca M., Knorr A., Grichois M.L., Et al., Erythrocytic sodium ion transport systems in primary hypertension in the rat, Kidney Int, 21, pp. S69-S75, (1982)
[4] Rosati C., Meyer P., Garay R., Sodium transport kinetics in erythrocyte from spontaneously hypertensive rats, Hypertension, 11, pp. 41-48, (1988)
[5] Cacciafesta M., Ferri C., Scuteri A, et al: 23Na NMR evaluation of human erythrocytes Na-K-Cl cotransport. A study in elderly hypertensives, Cell Mol Biol, 38, 8, pp. 877-884, (1992)
[6] Bianchi G., Ferrari P., Cusi D., Et al., Genetic aspects of ion transport systems in hypertension, J Hypertens, 8, pp. S213-S217, (1990)
[7] Cusi D., Sodium cell metabolism and hypertension (In Italian), Ann Ital Med Int, 1, pp. 48-61, (1989)
[8] Garay R., Nazaret C., Hannaert P., Price M., Abnormal Na+/K+ cotransport function in a group of patients with essential hypertension, Eur J Clin Invest, 13, pp. 311-316, (1984)
[9] Tuck M.L., Gross R., Maxwell M.H., Erythrocyte Na+/K+ cotransport and Na+/K+ pump in black and Caucasian hypertensive patients, Hypertension, 6, pp. 536-544, (1984)
[10] Canessa M., Spalvins A., Adragna N., Falkner B., Red cell sodium countertransport and cotransport in normotensive and hypertensive blacks, Hypertension, 6, pp. 344-351, (1985)

← 1 2 3 4 →