PHAGOCYTOSIS ASSOCIATED CHEMILUMINESCENCE OF HEMOCYTES IN MYTILUS-EDULIS (BIVALVIA)

被引：73
作者：
NOEL, D
BACHERE, E
MIALHE, E
机构：
[1] IFREMER, CNRS/UM2, Défense et Résistance chez les Invertébrés Marins, F-34095 Montpellier Cédex 5
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1993年 / 17卷 / 06期
关键词：
MOLLUSK; MYTILUS; HEMOCYTES; PHAGOCYTOSIS; RESPIRATORY BURST; CHEMILUMINESCENCE; OXYGEN RADICALS; MONOCLONAL ANTIBODY; ISOPYCNIC CENTRIFUGATION; SUBPOPULATION;
D O I：
10.1016/S0145-305X(05)80004-7
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The respiratory burst associated with phagocytosis by Mytilus edulis hemocytes was investigated by measurement of luminol-dependent chemiluminescence (LDCL). After experimental parameters (number of cells, quantity of stimulus) were determined, the biochemical mechanisms involved in the chemiluminescent process were investigated using inhibitors of oxygen radicals and enzymes. In particular, catechol-like phenols suggested the involvement of NADPH-oxidase and peroxidase in oxidative metabolism of mussel hemocytes. The variability of LDCL response observed among individuals and separated hemocyte subpopulations strongly suggests a variable immunocapacity depending on hemogram composition. Using a specific monoclonal antibody to discriminate different hemocyte types, the eosinophilic granulocytes appeared to exhibit the highest LDCL activity.
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收藏
页码：483 / 493
页数：11
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