PURIFICATION AND CRYSTALLIZATION OF HUMAN CYCLIN-DEPENDENT KINASE-2

被引：53
作者：
ROSENBLATT, J
DEBONDT, H
JANCARIK, J
MORGAN, DO
KIM, SH
机构：
[1] UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
[2] LAWRENCE BERKELEY LAB, BERKELEY, CA 94720 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 04期
关键词：
CELL CYCLE; CYCLIN DEPENDENT KINASE; PROTEIN KINASE; PROTEIN CRYSTALLIZATION; PROTEIN PURIFICATION;
D O I：
10.1006/jmbi.1993.1248
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis. © 1993 Academic Press, Inc.
引用
收藏
页码：1317 / 1319
页数：3
相关论文
共 23 条
  • [1] ACTIVATION OF HUMAN CYCLIN-DEPENDENT KINASES INVITRO
    DESAI, D
    GU, Y
    MORGAN, DO
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1992, 3 (05) : 571 - 582
  • [2] ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE
    DULIC, V
    LEES, E
    REED, SI
    [J]. SCIENCE, 1992, 257 (5078) : 1958 - 1961
  • [3] CDK2 ENCODES A 33-KDA CYCLIN-A-ASSOCIATED PROTEIN-KINASE AND IS EXPRESSED BEFORE CDC2 IN THE CELL-CYCLE
    ELLEDGE, SJ
    RICHMAN, R
    HALL, FL
    WILLIAMS, RT
    LODGSON, N
    HARPER, JW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) : 2907 - 2911
  • [4] EVIDENCE THAT THE G1-S AND G2-M TRANSITIONS ARE CONTROLLED BY DIFFERENT CDC2 PROTEINS IN HIGHER EUKARYOTES
    FANG, F
    NEWPORT, JW
    [J]. CELL, 1991, 66 (04) : 731 - 742
  • [5] CYCLIN-A IS REQUIRED FOR THE ONSET OF DNA-REPLICATION IN MAMMALIAN FIBROBLASTS
    GIRARD, F
    STRAUSFELD, U
    FERNANDEZ, A
    LAMB, NJC
    [J]. CELL, 1991, 67 (06) : 1169 - 1179
  • [6] TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS
    GOULD, KL
    NURSE, P
    [J]. NATURE, 1989, 342 (6245) : 39 - 45
  • [7] PHOSPHORYLATION AT THR167 IS REQUIRED FOR SCHIZOSACCHAROMYCES-POMBE P34CDC2 FUNCTION
    GOULD, KL
    MORENO, S
    OWEN, DJ
    SAZER, S
    NURSE, P
    [J]. EMBO JOURNAL, 1991, 10 (11) : 3297 - 3309
  • [8] CELL-CYCLE REGULATION OF CDK2 ACTIVITY BY PHOSPHORYLATION OF THR160 AND TYR15
    GU, Y
    ROSENBLATT, J
    MORGAN, DO
    [J]. EMBO JOURNAL, 1992, 11 (11) : 3995 - 4005
  • [9] HANKS SK, 1991, METHOD ENZYMOL, V200, P38
  • [10] FORMATION AND ACTIVATION OF A CYCLIN E-CDK2 COMPLEX DURING THE G(1)-PHASE OF THE HUMAN CELL-CYCLE
    KOFF, A
    GIORDANO, A
    DESAI, D
    YAMASHITA, K
    HARPER, JW
    ELLEDGE, S
    NISHIMOTO, T
    MORGAN, DO
    FRANZA, BR
    ROBERTS, JM
    [J]. SCIENCE, 1992, 257 (5077) : 1689 - 1694
123