PURIFICATION AND CRYSTALLIZATION OF HUMAN CYCLIN-DEPENDENT KINASE-2

被引：53
|
作者：
ROSENBLATT, J
DEBONDT, H
JANCARIK, J
MORGAN, DO
KIM, SH
机构：
[1] UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
[2] LAWRENCE BERKELEY LAB, BERKELEY, CA 94720 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 04期
关键词：
CELL CYCLE; CYCLIN DEPENDENT KINASE; PROTEIN KINASE; PROTEIN CRYSTALLIZATION; PROTEIN PURIFICATION;
D O I：
10.1006/jmbi.1993.1248
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis. © 1993 Academic Press, Inc.
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页码：1317 / 1319
页数：3
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