COMPARISON OF METHODS USED FOR DETECTION OF MYCOPLASMA CONTAMINATION IN CELL-CULTURES, SERA, AND LIVE-VIRUS VACCINES

被引：0
作者：
BENISHEVA, T
SOVOVA, V
IVANOV, I
OPALCHENOVA, G
机构：
[1] ACAD SCI CZECH REPUBL, INST MOLEC GENET, CS-16637 PRAGUE, CZECHOSLOVAKIA
[2] BULGARIAN ACAD SCI, INST GEN & COMPARAT PATHOL, BU-1113 SOFIA, BULGARIA
来源：
FOLIA BIOLOGICA | 1993年 / 39卷 / 05期
关键词：
MYCOPLASMA; DETECTION TECHNIQUES; CONTAMINATION OF CELL CULTURE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.
引用
收藏
页码：270 / 275
页数：6
相关论文
共 24 条
  • [1] BARILE MF, 1981, ISRAEL J MED SCI, V17, P555
  • [2] FAST AND SIMPLE PROCEDURE FOR THE DETECTION OF CELL-CULTURE MYCOPLASMAS USING A SINGLE MONOCLONAL-ANTIBODY
    BLAZEK, R
    SCHMITT, K
    KRAFFT, U
    HADDING, U
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) : 203 - 212
  • [3] Del Guidice RA, 1978, MYCOPLASMA INFECTION, P57
  • [4] FREUND E, 1979, METHOD MICROBIOL, P391
  • [5] MYCOPLASMA-INFECTION OF CULTURED-CELLS
    HAY, RJ
    MACY, ML
    CHEN, TR
    [J]. NATURE, 1989, 339 (6224) : 487 - 488
  • [6] HAYFLICK L, 1965, TEX REP BIOL MED, VS 23, P285
  • [7] PROBLEMS CONCERNING NONCULTIVABLE MYCOPLASMA CONTAMINANTS IN TISSUE-CULTURES
    HOPPS, HE
    MEYER, BC
    BARILE, MF
    DELGIUDI.RA
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1973, 225 (DEC14) : 265 - 276
  • [8] IVANOV I, 1991, GEN COMP PATHOL, V28, P19
  • [9] JURMANOVA K, 1986, ARCH EXP VET MED, V40, P136
  • [10] KOTANI H, 1990, IN VITRO CELL DEV B, V26, P91
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