INJURY-INDUCED CHANGES IN CYTOSOLIC CA-2+ IN INDIVIDUAL RABBIT PROXIMAL TUBULE CELLS

被引:31
作者
SMITH, MW
PHELPS, PC
TRUMP, BF
机构
[1] UNIV MARYLAND,SCH MED,DEPT PATHOL,10 S PINE ST,BALTIMORE,MD 21201
[2] MARYLAND INST EMERGENCY MED SERV SYST,BALTIMORE,MD 21201
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 04期
关键词
ANOXIA; BLEBBING; CALCIUM IONOPHORES; CELL INJURY; CYTOSOLIC CALCIUM; N-ETHYLMALEIMIDE; NEPHROTOXICITY; PARA-CHLOROMERCURIBENZENE; PARA-CHLOROMERCURIBENZENE SULFONATE; TRYPSIN;
D O I
10.1152/ajprenal.1992.262.4.F647
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Cell injury was studied in cultured rabbit proximal tubular epithelial cells using digital-imaging fluorescent microscopy to relate changes in cytosolic Ca2+ ([Ca2+]i) to bleb formation and cell death. Fura-2-loaded cells were treated in normal (1.37 mM) and low (< 5-mu-M) extracellular Ca2+ ([Ca2+]e) with 1) inhibitors of glycolysis (iodoacetate) and/or mitochondrial oxidation (KCN), 2) thiol-modifying reagents (N-ethylmaleimide, p-chloromercuribenzene, and p-chloromercuribenzene sulfonate), and 3) Ca2+ ionophore (ionomycin). All three types of injury produced both [Ca2+]e-independent and [Ca2+]e-dependent increases in [Ca2+]i. KCN + iodoacetate +/- [Ca2+]e did not produce blebbing or death within 60-90 min. Thiol modifiers and ionomycin produced blebbing, which correlated with sustained threefold or greater elevations of [Ca2+]i and loss of viability only after [Ca2+]i had risen severalfold. Blebbing and cell death could be prevented or delayed by treatment in low [Ca2+]e. Trypsin (x 0.5) caused a transient (< 5 min) elevation in [Ca2+]i as well as increases in intracellular Ca2+ pools.
引用
收藏
页码:F647 / F655
页数:9
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