Synthesis and sequential separation of Co (II), Mn (II) and Cu (II) metal complexes using diazepam (7-Chloro-1methyl-5-phenyl-3H-1,4-benzodiapine-2-one) on high performance liquid chromatography

Present work describe the synthesis, characterization and sequential separation of metal complexes have been characterised by elemental analysis & IR spectral data. Diazepam forms stable anionic complexes with a wide range of metal ions. These ions can be separated qualitatively by reverse phase chromatography converting C-18 stationary phase coloumn to ion exchange one with an eluent containing acetonitrile water & appropriate amount of Diazepam. In this system 0.05 mol L-1 Diazepam is prepared by dissolving 0.0142 gm in the mobile phase & the pH is adjusted to 8 by NH3 buffer. Because of the intense UV absorption in the low UV range, detection at 254 nm is recommended for Diazepam complexes. The developed method simultaneously separated the above Co, Mn and Cu Diazepam complexes.