PHYSIOLOGICAL-STUDIES ON THE UTILIZATION OF OLEIC-ACID BY SACCHAROMYCES-CEREVISIAE IN RELATION TO MICROBODY DEVELOPMENT

被引：19

作者：

EVERS, ME

HOHFELD, J

KUNAU, WH

HARDER, W

VEENHUIS, M

机构：

[1] UNIV GRONINGEN, CTR BIOL, ELECTRON MICROSCOPY LAB, KERKLAAN 30, 9751 NN HAREN, NETHERLANDS

[2] RUHR UNIV BOCHUM, FAC MED, INST PHYSIOL CHEM, W-4630 BOCHUM, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 90卷 / 01期

关键词：

PEROXISOMES; PEROXISOME-DEFICIENT (PAS) MUTANT; OLEIC ACID; BETA-OXIDATION; CONTINUOUS CULTURES; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0378-1097(91)90649-U

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbody biogenesis/assembly in constructed, conditional pas mutants.

引用

页码：73 / 78

页数：6

共 19 条

[1]

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[2] PEROXISOMAL BETA-OXIDATION SYSTEM OF CANDIDA-TROPICALIS - PURIFICATION OF A MULTIFUNCTIONAL PROTEIN POSSESSING ENOYL-COA HYDRATASE, 3-HYDROXYACYL-COA DEHYDROGENASE AND 3-HYDROXYACYL-COA EPIMERASE ACTIVITIES [J].