CHROMOSOMAL DNA PATTERNS AND MITOCHONDRIAL-DNA POLYMORPHISM AS TOOLS FOR IDENTIFICATION OF ENOLOGICAL STRAINS OF SACCHAROMYCES-CEREVISIAE

Chromosomal DNA patterns using the transverse alternating field electrophoresis technique and mitochondrial DNA restriction profiles have been achieved for 22 enological strains of Saccharomyces cerevisiae. Both methods have evidenced a marked polymorphism of these strains. Twenty different karyotypes and 17 mitochondrial DNA banding patterns have been observed. Only three strains originating from the same vineyard could not be differentiated by either of the two methods. The polymorphism observed at the chromosomal and mitochondrial levels makes the techniques investigated powerful tools for identification and control of industrial strains. © 1990 Springer-Verlag.