DETECTION BY WESTERN BLOTTING OF AN ANTIBODY TO THE HEPATITIS-C VIRUS E1 ENVELOPE PROTEIN IN SERA OF PATIENTS WITH CHRONIC LIVER-DISEASE

被引：0

作者：

HADA, H ^{[1
]}

KOIDE, N ^{[1
]}

HANAFUSA, T ^{[1
]}

SAKAGUCHI, K ^{[1
]}

SHINJI, T ^{[1
]}

SASAKI, S ^{[1
]}

OKA, T ^{[1
]}

TAKAYAMA, N ^{[1
]}

YUMOTO, Y ^{[1
]}

TSUJI, T ^{[1
]}

机构：

[1] OKAYAMA UNIV, RADIOISOTOPE RES CTR, OKAYAMA 700, JAPAN

来源：

ACTA MEDICA OKAYAMA | 1992年 / 46卷 / 05期

关键词：

HEPATITIS-C VIRUS; ENVELOPE; ANTIBODY; WESTERN BLOTTING;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of El envelope protein and beta-galactosidase as an antigen. The antibody to HCV El (anti-HCV El) was detected in 8 (42 %) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17 %) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89 %) of 9 anti-HCV El positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.

引用

页码：365 / 370

页数：6

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