IMPACT OF THE MUNICIPAL LANDFILL SITE ON BACTERIA PARTICIPATING IN TRANSFORMATION OF SOIL NITROGEN

Field research on the subject presented in the paper were conducted from March 2006 until September 2007. For the experimental reasons 8 research plots for soil sampling were established on each side of the municipal waste landfill site in Tarnow in two zones: 50-200 and 250-500 m from its boundaries. Spring wheat, Zura c.v. was cultivated on the plots. An additional experimental plot was set up in the reclaimed part of the landfill site. Analysis of the obtained results points to differences in the quantitative composition of microflora participating in nitrogen metabolism in the analyzed soils. It was found that on the experimental plots under wheat the number of proteolytic bacteria in 1 g of the soil dry mass ranged from 2 300 to 96 700 cfu, ammonifying bacteria from 122 000 to 4 860 000 and bacteria from Azotobacter genus from 0 to 330 cfu. Over the whole period of investigations also Clostridium pasteurianum bacteria counts were determined within the range of 0.01 to 0.00001, the values of nitrification process index from 0.01 to 0.000001 and denitrification index from 0.001 to 0.00001 were assessed in the whole analyzed soil environment.