Physical Mapping of the Cystic Fibrosis Region by Pulsed-Field Gel Electrophoresis

被引：42

作者：

Drumm, Mitchell L. ^{[1
,2
,3
]}

Smith, Casandra L. ^{[4
,5
]}

Dean, Michael ^{[6
]}

Cole, Jeffery L. ^{[1
,2
,3
]}

Iannuzzi, Michael C. ^{[1
,2
,3
]}

Collins, Francis S. ^{[1
,2
,3
]}

机构：

[1] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA

[4] Columbia Univ Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

[5] Columbia Univ Coll Phys & Surg, Dept Psychiat, New York, NY 10032 USA

[6] Program Resources Inc, Biol Carcinogenesis Dev Program, Frederick Canc Res Facil, Frederick, MD 21701 USA

来源：

GENOMICS | 1988年 / 2卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0888-7543(88)90024-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene for cystic fibrosis (CF) is known to be flanked by the closely linked DNA markers met and J3.11 on chromosome 7. Using the technique of pulsed-field gel electrophoresis, we have constructed a complete overlapping restriction map of approximately 3000 kb of DNA in this region. The met and J3.11 probes are found to be between 1300 and 1800 kb apart, which compares well with their genetic distance of 1-2 cM. The CF gene must be located within this interval, and the availability of this physical map should be of considerable utility in mapping additional clones as the search for the gene proceeds. (C) 1989 Academic Press, Inc.

引用

页码：346 / 354

页数：9

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