IDENTIFICATION AND CHARACTERIZATION OF A 2ND POLYGALACTURONASE GENE OF ASPERGILLUS-NIGER

被引：72

作者：

BUSSINK, HJD ^{[1
]}

BROUWER, KB ^{[1
]}

DEGRAAFF, LH ^{[1
]}

KESTER, HCM ^{[1
]}

VISSER, J ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT GENET,MOLEC GENET SECT,6703 HA WAGENINGEN,NETHERLANDS

来源：

CURRENT GENETICS | 1991年 / 20卷 / 04期

关键词：

PECTIN DEGRADATION; FILAMENTOUS FUNGUS; INTRONS; OVEREXPRESSION;

D O I：

10.1007/BF00318519

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The filamentous fungus Aspergillus niger produces several endopolygalacturonases that are involved in the degradation of pectin. PGI, the enzyme representing the second most abundant activity in a commercial enzyme preparation, was further characterized and the corresponding gene was isolated. The nucleotide sequence of the pgaI gene was determined and the protein coding region was found to be interrupted by two short introns, one of which has a unusual donor splice site. The deduced 368 amino acids long protein with a putative prepropeptide of 31 amino acids shows 60% sequence identity to PGII in the mature protein. PGI overproducing A. niger strains were obtained by cotransformation with the cloned gene.

引用

页码：301 / 307

页数：7

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