TRANSLATION OF TYPE-II PROCOLLAGEN MESSENGER-RNA AND HYDROXYLATION OF THE CELL-FREE PRODUCT

被引：22

作者：

CHEAH, KSE

GRANT, ME

JACKSON, DS

机构：

[1] Department of Medical Biochemistry, University of Manchester Medical School, Manchester, M13 9PT, Oxford Road

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91982-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(A)-rich RNA was isolated from embryonic chick sternal cartilage cells and translated in a rabbit reticulocytelysate messenger-dependent cell-free protein synthesizing system. The presence of mRNA for procollagen type II was indicated by the synthesis of a high molecular weight collagenous polypeptide. This component is identified as the unhydroxylated precursor of type II procollagen on the basis of its electrophoretic mobility, its susceptibility to highly purified bacterial collagenase, and its ability to act as a substrate for collagen prolyl hydroxylase. © 1979.

引用

页码：1025 / 1031

页数：7

共 15 条

[1]

ADAMS SL, 1979, J BIOL CHEM, V254, P4935

[2] TRANSLATION OF EMBRYONIC-CHICK TENDON PROCOLLAGEN MESSENGER RIBONUCLEIC-ACID IN 2 CELL-FREE PROTEIN-SYNTHESIZING SYSTEMS [J].

CHEAH, KSE ;

GRANT, ME ;

JACKSON, DS .

BIOCHEMICAL JOURNAL, 1979, 182 (01) :81-93

[3] BIOSYNTHESIS OF CARTILAGE PROCOLLAGEN [J].

DEHM, P ;

PROCKOP, DJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 35 (01) :159-166

[4]

GRAVES PN, 1979, FED PROC, V38, P620

[5] MODIFIED PROCEDURE FOR ASSAY OF H3-OR C14-LABELED HYDROXYPROLINE [J].

JUVA, K ;

PROCKOP, DJ .

ANALYTICAL BIOCHEMISTRY, 1966, 15 (01) :77-&

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] CONSTRUCTION AND CHARACTERIZATION OF PRO ALPHA-1 COLLAGEN COMPLEMENTARY DEOXYRIBONUCLEIC-ACID CLONES [J].

LEHRACH, H ;

FRISCHAUF, AM ;

HANAHAN, D ;

WOZNEY, J ;

FULLER, F ;

BOEDTKER, H .

BIOCHEMISTRY, 1979, 18 (14) :3146-3152

[8]

MUIR H, 1975, MTP INT REV SCI BIOC, V5, P153

[9]

PALMITER RD, 1979, J BIOL CHEM, V254, P1433

[10] EFFICIENT MESSENGER-RNA-DEPENDENT TRANSLATION SYSTEM FROM RETICULOCYTE LYSATES [J].

PELHAM, HRB ;

JACKSON, RJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 67 (01) :247-256

← 1 2 →