THE MONOCARBOXYLATE CARRIER FROM RAT-LIVER MITOCHONDRIA - PURIFICATION AND KINETIC CHARACTERIZATION IN A RECONSTITUTED SYSTEM

被引：15

作者：

CAPUANO, F

DIPAOLA, M

AZZI, A

PAPA, S

机构：

[1] UNIV BARI,INST MED BIOCHEM & CHEM,I-70124 BARI,ITALY

[2] UNIV BERN,INST BIOCHEM & MOLEK BIOL,CH-3012 BERN,SWITZERLAND

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

(Rat liver); Mitochondria; Monocarboxylate carrier; Pyruvate transport; Reconstitution;

D O I：

10.1016/0014-5793(90)80631-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The monocarboxylate (pyruvate) carrier was extracted from rat liver mitochondria with Triton X-100 in the presence of asolectin and partially purified by chromatography on HTP. The HTP eluate reconstituted in liposomes was shown to catalyze active pyruvatein/acetoacetateout, and acetoacetatein/pyruvateout counter-exchange. Kinetic characterization of the reconstituted pyruvate carrier was achieved by an original spectrophotometric method consisting of determination of substrate release from proteoliposomes with a coupled enzymatic assay. © 1990.

引用

页码：39 / 42

页数：4

共 21 条

[1]

AZZI A, 1989, ANION CARRIERS MITOC

[2] PURIFICATION AND RECONSTITUTION OF 2 ANION CARRIERS FROM RAT-LIVER MITOCHONDRIA - THE DICARBOXYLATE AND THE 2-OXOGLUTARATE CARRIER [J].