THE MONOCARBOXYLATE CARRIER FROM RAT-LIVER MITOCHONDRIA - PURIFICATION AND KINETIC CHARACTERIZATION IN A RECONSTITUTED SYSTEM

被引：15
|
作者：
CAPUANO, F
DIPAOLA, M
AZZI, A
PAPA, S
机构：
[1] UNIV BARI,INST MED BIOCHEM & CHEM,I-70124 BARI,ITALY
[2] UNIV BERN,INST BIOCHEM & MOLEK BIOL,CH-3012 BERN,SWITZERLAND
来源：
FEBS LETTERS | 1990年 / 261卷 / 01期
关键词：
(Rat liver); Mitochondria; Monocarboxylate carrier; Pyruvate transport; Reconstitution;
D O I：
10.1016/0014-5793(90)80631-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The monocarboxylate (pyruvate) carrier was extracted from rat liver mitochondria with Triton X-100 in the presence of asolectin and partially purified by chromatography on HTP. The HTP eluate reconstituted in liposomes was shown to catalyze active pyruvatein/acetoacetateout, and acetoacetatein/pyruvateout counter-exchange. Kinetic characterization of the reconstituted pyruvate carrier was achieved by an original spectrophotometric method consisting of determination of substrate release from proteoliposomes with a coupled enzymatic assay. © 1990.
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页码：39 / 42
页数：4
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