DISSOCIATION OF RABBIT RETICULOCYTE RIBOSOMES WITH EDTA AND LOCATION OF MESSENGER RIBONUCLEIC ACID

被引：19
作者：
NOLAN, RD
ARNSTEIN, HR
机构：
[1] Department of Biochemistry University of London King's College Strand, London
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 04期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00629.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rabbit reticulocyte ribosomes were incubated with polyuridylic acid at 37° for 1 h under conditions suitable for protein synthesis. Ribosomes containing bound polyuridylic acid were isolated and dissociated into subparticles by treatment with EDTA. The subparticles were separated by zone centrifugation in a sucrose density gradient and the distribution of polyuridylic acid was examined by measuring the ability of gradient fractions to stimulate the incorporation of [14C] phenylalanine into protein by a cell‐free system. It was observed that the peak of polyuridylic acid activity corresponded to a region of the subparticle gradient having a sedimentation coefficient of about 50 S. When [14C] polyuridylic acid was used in the initial binding reaction, subsequent analysis of subparticles produced by treatment with EDTA confirmed the presence of polyuridylic acid on or close to the large ribosomal subparticle. Copyright © 1969, Wiley Blackwell. All rights reserved
引用
收藏
页码：445 / &
相关论文
共 22 条
123