NEW METHOD FOR THE EXTRACTION OF VIRAL-RNA AND DNA FROM CEREBROSPINAL-FLUID FOR USE IN THE POLYMERASE CHAIN-REACTION ASSAY

被引：196

作者：

CASAS, I ^{[1
]}

POWELL, L ^{[1
]}

KLAPPER, PE ^{[1
]}

CLEATOR, GM ^{[1
]}

机构：

[1] UNIV MANCHESTER,SCH MED,DEPT PATHOL SCI,DIV VIROL,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 01期

关键词：

RNA EXTRACTION; DNA EXTRACTION; POLYMERASE CHAIN REACTION; ENTEROVIRUS; HUMAN HERPES VIRUS;

D O I：

10.1016/0166-0934(94)00173-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, rapid, and simple method for the isolation of either RNA or DNA from cerebrospinal fluid samples for subsequent amplification by specific polymerase chain reaction (PCR) assays is described. The technique involves a single extraction with a guanidinium thiocyanate acid (GuSCN) buffer, and does not require the use of organic solvents. Applied to the recovery of enteroviral RNA, herpes simplex virus (HSV) and Varicella-zoster virus (VZV) DNAs the method has proved to be of equivalent or better efficiency than established methods of nucleic acid separation but is less laborious and time consuming. The simplicity of the procedure permits the processing of large numbers of samples and the use of a single preparative method for either RNA or DNA PCR makes it an attractive method for the routine laboratory.

引用

页码：25 / 36

页数：12

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