THE X-RAY STRUCTURE OF A TETRAPEPTIDE BOUND TO THE ACTIVE-SITE OF HUMAN CYCLOPHILIN-A

被引：124

作者：

KALLEN, J ^{[1
]}

WALKINSHAW, MD ^{[1
]}

机构：

[1] SANDOZ PHARMA AG,DRUG DESIGN GRP 503 558,PRECLIN RES,CH-4002 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 300卷 / 03期

关键词：

X-RAY STRUCTURE; CIS-TRANS ISOMERASE; CYCLOPHILIN;

D O I：

10.1016/0014-5793(92)80865-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human cyclophilin A (165 residues) has peptidyl-prolyl cis-trans isomerase activity. Here we report a high-resolution three-dimensional X-ray structure of a substrate, ac-Ala-Ala-Pro-Ala-amc (ac, acetyl; amc, amidomethylcoumarin) bound to the active-site of cyclophilin. The structure consisting of a dimer of complexes and 135 water molecules was refined to a crystallographic R-factor of 17.7% for all data in the range 8 angstrom-2.3 angstrom.

引用

页码：286 / 290

页数：5

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