USE OF ELISA TO DETECT TOXIGENIC PASTEURELLA-MULTOCIDA IN ATROPHIC RHINITIS IN SWINE

被引：8

作者：

BOWERSOCK, TL

HOOPER, T

POTTENGER, R

机构：

[1] Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, W. Lafayette

[2] Animal Disease Diagnostic Laboratory, Southern Indiana Purdue Agricultural Center, Dubois

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1992年 / 4卷 / 04期

关键词：

D O I：

10.1177/104063879200400409

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermonecrotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.

引用

页码：419 / 422

页数：4

共 21 条

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