NMR-STUDIES OF A DNA CONTAINING 8-HYDROXYDEOXYGUANOSINE

被引：210

作者：

ODA, Y

UESUGI, S

IKEHARA, M

NISHIMURA, S

KAWASE, Y

ISHIKAWA, H

INOUE, H

OHTSUKA, E

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,1-6 YAMADAOKA,SUITA,OSAKA 565,JAPAN

[2] PROT ENGN RES INST,SUITA,OSAKA 565,JAPAN

[3] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

[4] NATL CANC CTR,RES INST,DIV BIOL,CHUO KU,TOKYO 104,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 07期

关键词：

D O I：

10.1093/nar/19.7.1407

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.

引用

页码：1407 / 1412

页数：6

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