NMR-STUDIES OF A DNA CONTAINING 8-HYDROXYDEOXYGUANOSINE

被引：213
作者：
ODA, Y
UESUGI, S
IKEHARA, M
NISHIMURA, S
KAWASE, Y
ISHIKAWA, H
INOUE, H
OHTSUKA, E
机构：
[1] OSAKA UNIV,FAC PHARMACEUT SCI,1-6 YAMADAOKA,SUITA,OSAKA 565,JAPAN
[2] PROT ENGN RES INST,SUITA,OSAKA 565,JAPAN
[3] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN
[4] NATL CANC CTR,RES INST,DIV BIOL,CHUO KU,TOKYO 104,JAPAN
来源：
NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 07期
关键词：
D O I：
10.1093/nar/19.7.1407
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.
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页码：1407 / 1412
页数：6
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