SENSITIVE DETECTION AND STRUCTURAL CHARACTERIZATION OF TRIMETHYL(P-AMINOPHENYL)AMMONIUM-DERIVATIZED OLIGOSACCHARIDES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY

被引：72

作者：

OKAMOTO, M

TAKAHASHI, KI

DOI, T

机构：

[1] Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd, Osaka, 554, 1–98, 3‐chome, Kasugade‐naka, Konohana‐ku

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 08期

关键词：

D O I：

10.1002/rcm.1290090804

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometric methodologies which use electrospray ionization (ESI) for oligosaccharide structural determination and which are linked to classical procedures, such as reductive amination, have been established. Several derivatives of a model oligosaccharide were prepared and their ionization efficiencies in electrospray ionization (ESI) and fast-atom bombardment (FAB) mass spectrometry were investigated. The trimethyl-(p-aminophenyl)ammonium (TMAPA) derivatives showed extremely high sensitivity in positive-ion ESI-MS and gave Y- and Z-series sequence ions by ESI tandem mass spectromety (MS/MS). This study suggests that TMAPA-derivatized oligosaccharides used in ESI-MS and ESI-MS/MS would be effective for structural characterization of oligosaccharides that are available only in limited quantities.

引用

页码：641 / 643

页数：3

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