GENETIC-MAPPING OF A QUANTITATIVE TRAIT LOCUS (QTL) THAT ENHANCES THE SHOOT DIFFERENTIATION RATE IN HORDEUM-VULGARE L

被引：58

作者：

KOMATSUDA, T ^{[1
]}

ANNAKA, T ^{[1
]}

OKA, S ^{[1
]}

机构：

[1] TOKIMEC,OHTA KU,TOKYO 144,JAPAN

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 86卷 / 06期

关键词：

HORDEUM-VULGARE; GENETIC MAPPING; ISOZYMES; QUANTITATIVE TRAIT LOCI; SHOOT DIFFERENTIATION ABILITY;

D O I：

10.1007/BF00222661

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A quantitative trait locus (QTL) controlling shoot differentiation from immature embryo callus was identified by linkage analysis with morphological and isozyme markers in barley, Hordeum vulgare L. Immature embryos were isolated from cvs 'Azumamugi' (difficult to differentiate), 'Kanto Nakate Gold' (easy to differentiate), their hybrids (F1) and a backcross population derived from a cross 'Azumamugi' x F1. The embryos were cultured in vitro for callus initiation and subsequent shoot differentiation. The shoot differentiation rate was closely associated with ear type (v locus), isocitrate dehydrogenase isozyme (Idh-2), and esterase isozyme (Est-11). These markers were found to reside in a chromosome segment of approximately 30 cM on chromosome 2. Recombination frequency was 9.9% between v and a proposed QTL named ''Shd1'' (shoot differentiation), 11.5% between Idh-2 and Shd 1, and 21.3% between Est-11 and Shd1. All data showed the Idh-2, v, Shd1 and Est-11 loci to be arranged in this order from proximal to distal on the long arm of chromosome 2.

引用

页码：713 / 720

页数：8

共 36 条

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[2]

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